Test Information
Test offerings and prices are subject to change without notice. For the most up to date pricing information please contact the VDL.
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| Lab(desc) | Lab Test Name | Specimen Amount (Min Amount) | Shipping Conditions | Turn Around Time / Schedule | Comments | Test Fee | Method QL = Qualitative QN = Quantitative |
|---|---|---|---|---|---|---|---|
| Diagnostic Plans | Calf Diarrhea Panel (Multiplex PCR) | Fecal specimen (at least 5 gr), intestinal scraping, bacterial isolate, nucleic acid | Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. Do not submit fecal specimens in gloves, screw-cap tubes are preferred. | 2-3 days | This is a multiplex PCR assay. These test results are intended to be used as an aid in the diagnosis of Bovine cornonavirus, Bovine rotavirus group A, E. coli K99, Salmonella, and Cryptosporidium. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella or E. coli, aerobic culture can be requested and antimicrobial susceptibility performed. | $65.00 | Real-time PCR(QL) |
| Molecular Biology | Anaplasma marginale real-time PCR | Minimum 5-10 ml whole blood (EDTA) specimen, minimum 10 gr tissues (preferably spleen). | Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. | 1-2 working days / Monday through Friday | Anaplasmosis, caused by Anaplasma marginale, is one of the most prevalent tick-transmitted rickettsial diseases of cattle worldwide. This real-time PCR test has been validated to detect Anaplasma marginale in necropsied animals and blood samples. | $40.00 | Real-time PCR(QL) |
| Molecular Biology | Anaplasma/Theileria Tick Panel PCR (Bovine Only) | Minimum 5-10ml whole blood (EDTA), minimum 10gm tissues (preferably spleen). | Specimens are submitted in a well labeled, leak-proof container maintained at cold temperature during transport. | 1-2 working days/M-F | Anaplasma marginale and Theileria orientalis are two of the most prevalent tick-transmitted rickettsial and hemoprotozoan diseases of cattle worldwide. Infection with these hemoprotozoan microorganisms may cause anemia, lethargy, abortion, jaundice and death. They may also be detected in asymptomatic animals. | $69.00 | Real-time PCR (QL) |
| Molecular Biology | Bluetongue Virus (BTV) real-time PCR | 10 ml whole blood (EDTA), swab, tissue (spleen is the preferred organ, minimum 0.3 gr) or viral isolate | Please submit the specimens as soon as possible in refrigerated conditions. | 2 working days | This test has been developed to detect Bluetongue Virus (BTV) in clinical specimens. | $40.00 | Real-time PCR(QL) |
| Molecular Biology | Bluetongue Virus (BTV) and Epizootic Hemorrhagic Disease Virus (EHDV) real-time PCR | 10 ml whole blood (EDTA), swab, tissue (minimum 0.3 gr) or viral isolate | Please submit the specimens as soon as possible in refrigerated conditions. | 2 working day | This test has been developed to detect both Bluetongue Virus (BTV) and Epizootic Hemorrhagic Disease Virus (EHDV) using a multiplex real-time PCR assay. | $60.00 | Real-time PCR(QL) |
| Molecular Biology | Bovine viral Diarrhea Virus RT-PCR (Real Time PCR) | 4-10 mL anticoagulated whole blood (EDTA blood), virus isolate, nucleic acid, ear notch, tissue (or scraping), milk, serum | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | $40.00 | Real-time PCR(QL) | |
| Molecular Biology | Chlamydiales PCR | Isolate (liquid suspension), swab or wash, or at least 200 mg fresh or fixed tissue. | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | This procedure will detect veterinary pathogens within the order Chlamydiales. | $35.00 | Real-time PCR(QL) |
| Molecular Biology | Clostridioides difficile (toxin A gene) PCR | 1 isolate (plate Bacterial isolate, scraping or feces. | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | $29.00 | PCR(QL) | |
| Molecular Biology | Clostridioides difficile (toxin B gene) PCR | 1 isolate (plate culture or liquid suspension) Bacterial isolate, scraping or feces. | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | $29.00 | PCR(QL) | |
| Molecular Biology | Clostridium perfringens Toxin Typing PCR | Bacterial isolate (plate culture or liquid suspension) | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | The C. perfringens genotyping polymerase chain reaction (PCR) test is a qualitative assay designed to determine the genotype based on which of the different major toxin genes are present in an isolate ? the test does not detect the presence of the toxin proteins. | $29.00 | PCR(QL) |
| Molecular Biology | Epizootic Hemorrhagic Disease virus (EHDV) real-time PCR | 10 ml whole blood (EDTA), swab, tissue (spleen is the preferred organ, minimum 0.3 gr) or viral isolate | Please submit the specimens as soon as possible in cold conditions. | 2 working days | This test has been developed to detect Epizootic Hemorrhagic Disease Virus (EHDV) in clinical specimens. | $40.00 | Real-time PCR(QL) |
| Molecular Biology | Foot and Mouth Disease - FMD (NAHLN Testing) | Swab (oral ), Vesicular fluid, epithelial tissue | Any questions regarding sampling techniques can be directed to the USDA-APHIS-VS/NVSL-FADDL Diagnostic Services Section, Phone: (631) 332-3256 OR (631) 332-6814 (after business hours). | 1-2 working days | $48.00 | Real-time PCR(QL) | |
| Molecular Biology | Leptospira spp. rPCR (Real Time PCR) | Bacterial isolate, 20-40 mL urine, 200 mg fresh tissue (Kidney, Liver, Placenta). Special Equine Urine Collection Instructions for Leptospiral Testing- Administer 10 mL, I/V Lasix? (furosemide) to the horse and allow the animal to urinate once before collection. Collect 20-40mL, mid-stream catch, of second voided urine and then immediately submit the specimen to the laboratory (within 2 hours of collection and before 2:00 pm if bacteriologic testing is also requested). | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | The Bacteriology section may request this test when the bacterium is isolated from clinical specimens. By design this assay detects only pathogenic Leptospira species. | $45.00 | Real-time PCR(QL) |
| Molecular Biology | Mycobacterium avium spp paratuberculosis (Johne’s) Individual | 3gm fresh feces in a clean, well-marked, leak proof container or fecal swab. | Store refrigerated. Ship with ice packs, do not freeze. | Mycobacterium avium subsp. paratuberculosis (MAP) is the agent responsible for Johne?s disease (chronic granulomatous enteritis of the small intestine). This incurable infection of cattle typically occurs in the first year of life; however, the clinical symptoms of the disease, persistent, nonresponsive diarrhea, may not appear for two years or more. | $46.00 | Real-time PCR(QL) | |
| Molecular Biology | Mycobacterium avium spp paratuberculosis (Johne’s) Pooled | Please submit fresh fecal samples or swabs in individual clean, well-marked, leak proof containers. *Pooling of up to 5 samples is available. Pooling will be done at the VDL. Samples should be clearly identified and pools noted. If the pool test yields a positive or inconclusive result, the samples comprising that pool will be tested individually ONLY if requested by submitter. | Store refrigerated. Ship with ice packs, do not freeze. | Mycobacterium avium subsp. paratuberculosis (MAP) is the agent responsible for Johne?s disease (chronic granulomatous enteritis of the small intestine). This incurable infection of cattle typically occurs in the first year of life; however, the clinical symptoms of the disease, persistent, nonresponsive diarrhea, may not appear for two years or more. | $51.00 | Real-time PCR(QL) |