Test Information

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Lab(desc) Lab Test Name Specimen Amount (Min Amount) Shipping Conditions Turn Around Time / Schedule Comments In State Fee Out of State Fee Method QL = Qualitative QN = Quantitative
Molecular Biology Equine Adenovirus PCR Viral isolate, 200 mg fresh tissues including placenta, brain, spinal cord, lymph node, spleen and liver or fixed tissue, scraping or feces, upper respiratory tract swabs. Contact the laboratory at 859-257-8283 before submitting any specimen not listed. respiratory tract washes; feces, intestinal scrapings and fecal swabs 1 - 2 working days $35.00 $52.50 PCR(QL)
Molecular Biology Equine Arteritis Virus rRT-PCR (EAV or EVA Real Time RT-PCR) 10 mL anticoagulated blood (EDTA), swab, wash, 200 mg tissue, 10 mL seminal fluid (see test notes), aspirate from upper respiratory tract; Two specimens for each animal tested are recommended (see test notes). Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1 - 2 working days Live Animal Testing: To optimize laboratory confirmation of a diagnosis of equine arteritis virus (EAV) infection, it is recommended that a nasopharyngeal swab or washing and an unclotted blood sample be obtained as early as possible following the onset of clinical signs or in suspect cases of unapparent EAV infection. At least 10 mL of blood should be collected into EDTA (or ACD but not heparin) anticoagulant. In suspected cases of EVA-related abortion, submit the entire fetus and placenta for laboratory examination. If submission of the entire fetus and placenta is not feasible, collect specimens of placental membranes, lung, spleen, thymus and liver into individual, leak-proof containers, clearly labeling each with animal ID, tissue and date of collection. Where a stallion is a suspect carrier of EAV, he should be collected and 10 mL of semen containing the sperm-rich fraction of the full ejaculate submitted for testing. While the PCR test may be performed on seminal fluids, however, for regulatory purposes, such specimens may also need to be tested by virus isolation. $31.00 $46.50 Real-time PCR(QL)
Molecular Biology Equine Diarrhea Panel (Multiplex PCR) - Bacterial Panel Minimum 50 gr fecal specimen (at least), intestinal scraping, bacterial isolate, nucleic acid Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. Do not submit fecal specimens in gloves, screw-cap tubes are preferred This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of Lawsonia intracellularis, Salmonella spp, Clostridium perfringens PCR , Clostridioides difficile PCR, Cryptosporidium, and Neorickettsia risticii (agent of potomac horse fever). Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella, aerobic culture can be requested and antimicrobial susceptibility performed. $86.00 $86.00 Real-time PCR (QL)
Molecular Biology Equine Diarrhea Panel (Multiplex PCR) - Combined (Bacterial and Viral) Panel Minimum 50 gr fecal specimen (at least), intestinal scraping, bacterial or viral isolate, nucleic acid Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. Do not submit fecal specimens in gloves, screw-cap tubes are preferred This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of Lawsonia intracellularis, Salmonella spp, Neorickettsia risticii (agent of potomac horse fever), Clostridium perfringens PCR, Clostridioides difficile PCR, Cryptosporidium, equine rotavirus and equine coronavirus. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. In case of positive results for Salmonella, aerobic culture can be requested and antimicrobial susceptibility performed. $160.00 $160.00 Real-time PCR (QL)
Molecular Biology Equine Diarrhea Panel (Multiplex PCR) - Viral Panel Minimum 50 gr fecal specimen (at least), intestinal scraping, viral isolate, nucleic acid Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. Do not submit fecal specimens in gloves, screw-cap tubes are preferred This is a multiplex Real-time PCR assay. These test results are intended to be used as an aid in the diagnosis of equine rotavirus and equine coronavirus. Results should be considered in conjunction with the quality of specimen submitted, clinical signs and animal history. $92.00 $92.00 Real-time PCR (QL)
Molecular Biology Equine Hepacivirus Real-time PCR Serum, plasma, and whole blood (EDTA, minimum 10 ml) liver, liver biopsy Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. 2 working days The University of Kentucky Veterinary Diagnostic Laboratory has validated an Equine Hepatitis real-time-PCR panel to detect Equine Parvovirus and Equine Hepacivirus in clinical samples. These assays can be performed either individually for Equine Parvovirus or Equine Hepacivirus or together as the Equine Hepatitis Panel. Both viruses have been reported to cause hepatitis in experimental horse models and have been associated with liver disease in naturally infected, clinically ill horses. As both viruses can be present in clinically normal horses, PCR results should be interpreted in conjunction with history, clinical findings, and other laboratory results. $37.00 $37.00 Real-time PCR (QL)
Molecular Biology Equine Herpesvirus 1 Detection (Real Time PCR-charged per animal) 10 mL blood (EDTA), swab, wash, or aspirate, 200 mg tissue; 2, 20 um slices from a paraffin embedded tissue block. Tissues include placenta, brain stem, brain, spinal cord, and lymph node Nucleic acid, Viral isolate; Swab, Wash, or aspirate from upper respiratory tract. Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1 - 2 working days Live Animal Testing: To optimize laboratory confimation of a diagnosis of equine herpesviris 1 (EHV1) infection, it is recommended that both a nasopharyngeal swab or washing and an unclotted blood sample be obtained as early as possible following the onset of clinical signs or in suspect cases of unapparent EHV infection. At least 10 mL of blood should be collected into EDTA (or ACD but not heparin) anticoagulant. The charge per animal for EHV1 PCR testing will be the same whether submitting a single specimen or the recommended pair of specimens. Postmortem Testing: For animals exhibiting signs suggestive of neurological herpesvirus submit well labeled fresh tissue, especially brain stem and submandibular lymph node tissue. For suspected EHV1 abortions submit the entire fetus and membranes. If not practical submit well labeled fresh tissue samples. The EHV1 pathotyping test can be performed for an additional fee on samples found to be positive using the EHV1 PCR test procedure. $48.00 $72.00 Real-time PCR(QL)
Molecular Biology Equine Herpesvirus 1 Pathotyping PCR (run only if Equine Herpesvirus 1 Detection PCR result is Positive) 10 mL blood (EDTA), 1 swab, wash, or aspirate, (200 mg tissue), (2, 20 um slices from a paraffin embedded tissue block.) Tissues include placenta, brain stem, brain, spinal cord, and lymph node Nucleic acid, Viral isolate; Swab, Wash, or aspirate from upper respiratory tract; fresh or fixed tissue. Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1 - 2 working days This test can be performed for an additional fee on samples found to be positive using the EHV1 PCR test procedure. $63.00 $95.50 Real-time PCR(QL)
Molecular Biology Equine Herpesvirus 2 PCR Swab, 10 mL anticoagulated blood (EDTA); wash, or aspirate, 200 mg tissue, 50 um slice from a paraffin embedded tissue block, Viral isolate; nucleic acid; Conjunctival Swab/scraping; also accepted are wash, or aspirate from upper respiratory tract; fresh tissue. Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1 - 2 working days $48.00 $72.00 PCR(QL)
Molecular Biology Equine Herpesvirus 3 PCR 200 mg tissue, Nucleic acid, Viral isolate; Swab or scraping from reproductive tract. Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1 - 2 working days For more information about the disease visit: tinyurl.com/merckehv $43.00 $64.00 PCR(QL)
Molecular Biology Equine Herpesvirus 4 PCR (Real Time PCR) 10 mL anticoagulated whole blood (EDTA), swab, wash, or aspirate, 200 mg tissue, 50 um slice from a paraffin embedded tissue block; pericardium or CSF body fluid accepted but not preferred; fresh tissues including placenta, brain, spinal cord, lymph node, spleen, and liver; fixed tissues; viral isolates; upper respiratory tract swabs; and upper respiratory tract washes. Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1 - 2 working days To optimize laboratory confirmation of a diagnosis of equine herpesvirus 4 (EHV4) infection, it is recommended that both a nasopharyngeal swab or washing and an unclotted blood sample be obtained as early as possible following the onset of clinical signs or in suspect cases of unapparent EHV infection. The charge per animal for EHV4 PCR testing will be the same whether submitting a single specimen or the recommended pair of specimens. For more information about the disease visit the Merck Manual website: https://www.merckvetmanual.com/ $48.00 $72.00 Real-time PCR(QL)
Molecular Biology Equine Herpesvirus 5 PCR 10 mL blood (EDTA); 1 swab, wash, or aspirate; at least 200 mg tissue; or at least a 50 um slice from a paraffin embedded tissue block Viral isolate; Swab, Wash, or aspirate from upper respiratory tract; anticoagulated whole blood (EDTA blood); fresh tissue. Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1 - 2 working days $44.00 $66.00 PCR(QL)
Molecular Biology Equine Influenza rRT-PCR (Real Time RT-PCR) Swab or Wash from upper respiratory tract. The recommended specimen type to submit is a nasal swab submerged in a viral transport medium packed in ice 1 - 2 working days The recommended specimen type to submit is a nasal swab submerged in a viral transport medium packed in ice. $48.00 $72.00 Real-time PCR(QL)
Molecular Biology Equine Parvovirus Real-time PCR Serum, plasma, and whole blood (EDTA, minimum 10 ml) liver, liver biopsy Specimens are submitted in a well labeled leak-proof container maintained at cold temperature during transport. 2 working days The University of Kentucky Veterinary Diagnostic Laboratory has validated an Equine Hepatitis real-time-PCR panel to detect Equine Parvovirus and Equine Hepacivirus in clinical samples. These assays can be performed either individually for Equine Parvovirus or Equine Hepacivirus or together as the Equine Hepatitis Panel. Both viruses have been reported to cause hepatitis in experimental horse models and have been associated with liver disease in naturally infected, clinically ill horses. As both viruses can be present in clinically normal horses, PCR results should be interpreted in conjunction with history, clinical findings, and other laboratory results. $37.00 $37.00 Real-time PCR (QL)
Molecular Biology Equine Protozoal Myeloencephalitis (Real Time PCR) 200 mg tissue, 50 um slice from a paraffin embedded tissue block, Brain, Spinal Cord, (CSF, anticoagulated blood(EDTA), may be attempted). Contact the laboratory at 859-257-8283 before submitting any specimen not listed. 1-2 working days This is a PCR test for Equine Protozoal Myeloencephalitis that only detects Sarcocystis neurona $38.00 $57.50 Real-time PCR(QL)