Test Information
Test offerings and prices are subject to change without notice. For the most up to date pricing information please contact the VDL.
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| Lab(desc) | Lab Test Name | Specimen Amount (Min Amount) | Shipping Conditions | Turn Around Time / Schedule | Comments | In State Fee | Out of State Fee | Method QL = Qualitative QN = Quantitative |
|---|---|---|---|---|---|---|---|---|
| Molecular Biology | Lawsonia intracellularis PCR | 5gr intestinal scraping, fecal specimen or fecal swab, tissue (intestinal mucosa scraping ? ileum), nucleic acid. | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | Please do not submit fecal specimens in gloves. Screw-cap tubes are preferred. | $48.00 | $72.00 | Real-time PCR (QL) |
| Molecular Biology | Leptospira spp. rPCR (Real Time PCR) | Bacterial isolate, 20-40 mL urine, 200 mg fresh tissue (Kidney, Liver, Placenta). Special Equine Urine Collection Instructions for Leptospiral Testing- Administer 10 mL, I/V Lasix? (furosemide) to the horse and allow the animal to urinate once before collection. Collect 20-40mL, mid-stream catch, of second voided urine and then immediately submit the specimen to the laboratory (within 2 hours of collection and before 2:00 pm if bacteriologic testing is also requested). | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | The Bacteriology section may request this test when the bacterium is isolated from clinical specimens. By design this assay detects only pathogenic Leptospira species. | $38.00 | $57.00 | Real-time PCR(QL) |
| Molecular Biology | Mycobacterium avium spp paratuberculosis (Johne’s) Individual | 3gm fresh feces in a clean, well-marked, leak proof container or fecal swab. | Store refrigerated. Ship with ice packs, do not freeze. | Mycobacterium avium subsp. paratuberculosis (MAP) is the agent responsible for Johne?s disease (chronic granulomatous enteritis of the small intestine). This incurable infection of cattle typically occurs in the first year of life; however, the clinical symptoms of the disease, persistent, nonresponsive diarrhea, may not appear for two years or more. | $46.00 | $69.00 | Real-time PCR(QL) | |
| Molecular Biology | Mycobacterium avium spp paratuberculosis (Johne’s) Pooled | Please submit fresh fecal samples or swabs in individual clean, well-marked, leak proof containers. *Pooling of up to 5 samples is available. Pooling will be done at the VDL. Samples should be clearly identified and pools noted. If the pool test yields a positive or inconclusive result, the samples comprising that pool will be tested individually ONLY if requested by submitter. | Store refrigerated. Ship with ice packs, do not freeze. | Mycobacterium avium subsp. paratuberculosis (MAP) is the agent responsible for Johne?s disease (chronic granulomatous enteritis of the small intestine). This incurable infection of cattle typically occurs in the first year of life; however, the clinical symptoms of the disease, persistent, nonresponsive diarrhea, may not appear for two years or more. | $46.00 | $69.00 | Real-time PCR(QL) | |
| Molecular Biology | Mycoplasma gallisepticum PCR | 1 isolate (liquid suspension), (200 mg fresh tissue, or swab), Mycoplasma culture isolate, Tracheal swab, Fresh tissue (lung) | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | $25.00 | $37.50 | Real-time PCR(QL) | |
| Molecular Biology | Mycoplasma species PCR | 1 isolate (liquid suspension) Mycoplasma culture isolate | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | The Bacteriology section may automatically request this test when the bacterium is isolated from clinical specimens. | $35.00 | $52.00 | PCR(QL) |
| Molecular Biology | Mycoplasma synoviae PCR | 1 isolate (liquid suspension), (200 mg fresh tissue, or swab) Mycoplasma culture isolate, Tracheal swab, Fresh tissue (lung) | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | $25.00 | $37.50 | PCR(QL) | |
| Molecular Biology | Nocardioform placentitis PCR panel | 1 placenta, swab, or isolate Bacterial isolates, placenta, and placenta swabs | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1-2 working days | In suspected cases of abortion due to mucoid nocardioform placentitis, submit the entire fetus and placenta for laboratory examination. If submission of the entire fetus and placenta is not feasible, collect swab specimens of placental membranes into individual, leak-proof containers, clearly labeling each with animal ID and date of collection. The margins of visable lesions should be sampled. Swabs should be tested by both aerobic culture and PCR analysis. | $57.00 | $57.00 | Real-time PCR (QL) |
| Molecular Biology | OvHV-2 (Malignant Catarrhal Fever) PCR | One isolate (liquid suspension), 10 mL anticoagulated blood (EDTA), (200 mg tissue) Viral isolate; anticoagulated whole blood (EDTA blood); fresh and fixed lymphoid tissue | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | $40.00 | $60.00 | PCR(QL) | |
| Molecular Biology | Potomac Horse Fever real-time PCR (charged per animal) | Submit a pair of specimens, 10 mL anticoagulated blood (EDTA) AND either feces (5 g) or a fecal swab. For postmortem testing about 0.5 g mucosal scraping | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | Two specimens for each animal tested are recommended for PCR analysis. The charge per animal for PCR testing will be the same whether submitting a single specimen or the recommended pair of specimens. For information regarding the disease visit: tinyurl.com/merckphf | $48.00 | $72.00 | Real-time PCR (QL) |
| Molecular Biology | Rhodococcus equi rPCR (Real Time PCR) | Nasal swab or wash, Abscess, swab, Bacterial isolate. | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | This test is designed to detect both ChoE gene that is universally conserved in R. equi and VapA gene that is present in virulent R. equi (plasmid gene). The Bacteriology section may request this test when the bacterium is isolated from clinical specimens. | $31.00 | $46.50 | Real-time PCR(QL) |
| Molecular Biology | Salmonella spp. rPCR (Real Time PCR) | 2-5 g feces, fecal swab, environmental swab, intestinal scraping, joint fluid, Bacterial isolate. () | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 2-3 working days | The Bacteriology section may request this test when the bacterium is isolated from clinical specimens. | $42.00 | $63.00 | Real-time PCR(QL) |
| Molecular Biology | Streptococcus equi PCR | Nasopharyngeal wash, swab, bacterial isolate, 10 mL anticoagulated blood(EDTA). Nasopharyngeal washings are recommended, nasopharyngeal swab, or lymph node abscess swab, or gutteral pouch swab, bacterial isolate or tissue (anticoagulated whole blood (EDTA) if bastard strangles is suspected). | Contact the laboratory at 859-257-8283 before submitting any specimen not listed. | 1 - 2 working days | $42.00 | $63.00 | PCR(QL) | |
| Molecular Biology | Theileria Duplex real-time pcr (Bovine Only) | Minimum 10ml whole blood (EDTA), minimum 10gm tissues (preferably spleen). | Specimens are submitted in a well labeled, leak-proof container maintained at cold temperature during transport. | 1-2 working days/M-F | This is a validated duplex real-time PCR that can detect T. orientalis and differentiate T. orientalis subtype Ikeda (the most associated subtype with clinical disease). Of note, there is no serum test available for diagnosis of Theileriosis due to Theileria orientalis. | $40.00 | $60.00 | Real-time PCR (QL) |
| Molecular Biology | Tritrichomonas fetus real-time PCR (Trich PCR) | inoculated trich pouch (InPouch), trich tube, direct smegma in PBS or sterile saline, feces (feline only) *There will be a minimum of a 24 hour incubation period for all samples. Samples should be sent at ambient temperatures (no ice packs). *Pooling of up to 5 samples is available (bovine only). Pooling will be done at the VDL. Samples should be clearly identified and pools noted. Please check link for more information and item number for InPouch https://biomeddiagnostics.com/prepared-culture-media/inpouch-tf-bovine | Please do not put ice bags inside the shipping box, since the organisms are sensitive to cold. | 3-4 working days | This test detects Tritrichomonas fetus in the genital tract of bovine and intestinal tract of cats. The test is able to differentiate nonpathogenic Trichomonas spp from T. fetus. | $37.00 | $55.50 | Real-time PCR(QL) |

